Organizational learning is a continuous process of integrating data and knowledge to ensure improvement. This qualitative study focuses on the starting points and presuppositions of organizational learning within a surgical department. Starting points for individual learning were informal and based on day-to-day work (e.g., solving a specific problem) and organizational learning encompassed more formal and intentional practices. Factors presupposing and framing the learning process can be divided into four categories: leadership and roles, practices and resources, collaboration and climate, and motivation and activity.