Physical and nonphysical violence can negatively impact the work environment, increase rates of burnout, and lower perceptions of patient safety. This study details workplace violence experienced and witnessed by perioperative anesthesiologist assistants, certified registered nurse anesthetists, physicians, and registered nurses. More than three-quarters of participants reported having experienced or witnessed some form of workplace violence. Less than half reported satisfaction with how the organization addressed and resolved the situation. The most common sources of workplace violence were the patient or a family member, friend, or physician in the perioperative environment.