Nurse staffing is an important factor in maintaining patient safety. In this study, the Nursing Home Survey on Patient Safety Culture was used to assess the association of registered nurse (RN) and certified nurse assistant (CNA) turnover on perceived patient safety culture. Results indicate that CNA turnover is associated with lower patient safety culture scores, but RN turnover is not. The authors conclude that patient safety culture improvements in nursing homes may be dependent on retaining a well-trained and skilled nursing staff.